Abstract
We present a versatile and compact laser system for nonlinear confocal microscopy consisting of an Er:fiber oscillator and an Yb:amplifier. This system provides free wavelength choice over nearly the entire visible spectrum as well as infrared femtosecond pulses at 775 nm and between 1050 nm and 1400 nm. A comparative analysis of DNA damage and repair after multi-photon absorption at 774 nm and 1050 nm is presented as an application example. Potential mechanisms underlying the difference in the observed spectrum of DNA lesions will be discussed.
© 2009 Optical Society of America
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