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Optica Publishing Group
  • Imaging and Applied Optics 2018 (3D, AO, AIO, COSI, DH, IS, LACSEA, LS&C, MATH, pcAOP)
  • OSA Technical Digest (Optica Publishing Group, 2018),
  • paper JTu4A.36
  • https://doi.org/10.1364/3D.2018.JTu4A.36

Lensless holographic microscope of biological samples

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Abstract

Lensless holographic microscopy shows high potential to be a practical point of care diagnostic tool due to its unique features like compactness, simple prototype, portability and on-chip operation. In the present work, a deep violet (370nm, 10 nm FWHM) LED at low power is used as a low coherent light source for lensless 3-D holographic imaging of live cells and microparticles. An on-chip Fresnel hologram of the sample is recorded in on-axis geometry on a high resolution CMOS camera and 3-D phase reconstruction has been performed eliminating the twin images using an optimization approach.

© 2018 The Author(s)

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