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Time-Sliced Transillumination Imaging of Normal and Cancerous Breast Tissues

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Abstract

Transillumination images of in vitro human female breast tissue specimens were recorded using an ultrafast electronic gated imaging camera system. Breast tissue specimens comprising normal and cancerous tissues were illuminated with 800 nm, approximately 130 fs, 1 kHz repetition-rate light pulses from a self-mode-locked Ti:sapphire laser and amplifier system. The gated imaging system formed a sequence of two-dimensional images using approximately 80-ps time slices of the transmitted light pulse. Images recorded with different time slices of transmitted light highlighted different types of tissues, and allowed distinguishing between normal and cancerous tissues. Transillumination imaging as a function of wavelength over the 1225–1300 nm range using a Crrforsterite laser demonstrated that the optical absorption band of adipose tissues around 1203 nm could be used as a ‘spectral finger print’ to obtain images of adipose tissues distinct from that of the fibrous tissues.

© 1998 Optical Society of America

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