Abstract
We present a method for quantitative tracking of phagosomes without labeling. Current imaging methods, used for phagosome research, have limitations in live-cell quantitative analysis. We reconstruct three-dimensional (3-D) refractive index (RI) tomograms from two-dimensional holograms with changing illumination angle. RI information enables to obtain intracellular phagosomes quantitatively. Time-lapse imaging provides 3-D information’s of phagosom movement. Collectively, we propose that quantitative phase imaging techniques can be a useful tool for phagocytosis research.
© 2014 Optical Society of America
PDF ArticleMore Like This
Seo Eun Lee, Kyoohyun Kim, Jonghee Yoon, Ji Han Heo, HyunJoo Park, Chulhee Choi, and YongKeun Park
ATh1I.3 Asia Communications and Photonics Conference (ACP) 2014
A. Su-A Yang, B. Jonghee Yoon, and C. YongKeun Park
27H2_5 Conference on Lasers and Electro-Optics/Pacific Rim (CLEO/PR) 2015
Matt Rinehart, Michael Giacomelli, Kevin Chalut, and Adam Wax
JMA7 Biomedical Optics (BIOMED) 2008